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Grading of recommendation as per OCEBM levels of evidence (March 2009)

2.4 Muscle proteome Frozen gastrocnemius tissue from the right hindlimb was crushed with a pestle and placed in homogenization buffer (1% Triton X-100, 50 mM HEPES, 12 mM Na pyrophosphate, 100 mM NaF, 10 mM EDTA, protease inhibitor, phosphatase inhibitor) with a stainless-steel bead

Quantitation of peptides and LCMS/MS analysis were performed by CPOS

This advanced protocol is not for beginners and requires careful monitoring and medical supervision

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However, their usage involves extensive ethical considerations, and the high costs and practical requirements can restrict their use in many studies