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et al.: Roles of dietary glycine, proline, and hydroxyproline in collagen synthesis and animal growth (2018) Amino Acids

A number of phenotypic elements should be carefully assessed, including neurological and cognitive symptoms (such as impairment of concentration and short-term memory, perceptual and sensory disturbances, sensory and emotional overload phenomena), autonomic manifestations (such as orthostatic intolerance, palpitations, gastro-intestinal symptoms), neuroendocrine manifestations (such as loss of thermostatic stability, intolerances of extremes of heat and cold, worsening symptoms with stress), and immune manifestations (such as tender lymph nodes, recurrent sore throat or flu-like symptoms, general malaise (flu-like feelings of being ill and feverish)

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Nature 27:442446 Foyer CH, Halliwell B (1976) The presence of glutathione and glutathione reductase in chloroplasts: a proposed role in ascorbic acid metabolism

Finally, elution buffers are added to disrupt the binding interaction, and release the target molecule, then collected in purified form